Destruction of the Dorsal Motor Nucleus of the Vagus Aggravates Inflammation and Injury from Acid-Induced Acute Esophagitis in a Rat Model

Figure 1

(a) Two weeks after DMV destruction under a light microscope: concurrent with axonal degeneration, myelin was disintegrated, which resulted in lipid and neutral fat, stained red-stained with Sultan III, activated microglia changed into phagocytic cells, necrotic neurons were devoured, bubble cells appeared, astrocytes proliferated, and ultimately honeycomb glial scars appeared. (b) Twenty-four hours after DMV destruction under a light microscope: the nuclei of nerve cells presented with pyknosis, the cells shrank and were deformed, Nissl bodies of the cytoplasm dissolved and disappeared, and then the cells dissolved and disappeared, creating so-called ghost cells that were deeply red-stained by HE. (c) c-fos-positive cells with red nuclei in the AM of all animals. Con: control group (×400); S+S: sham + saline group (×400); S+A: sham + acid group (×400); O+S: operation + saline group (×400); O+A: operation + acid group (×400). (d) c-fos expression in the AM increased more significantly in the sham + acid group than in the sham + saline group (). After DMV destruction, c-fos expression more significantly decreased in the AM in the operation + saline group than in the sham + acid group (). c-fos expression was much higher in the AM in the operation + acid group than in the operation + saline group () (, ).

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