The Laminin-α1 Chain-Derived Peptide, AG73, Binds to Syndecans on MDA-231 Breast Cancer Cells and Alters Filopodium Formation

Figure 4

Silencing the expression of Sdcs 1, 2, and 4 decreased filopodium formation in MDA-231 breast cancer cells. (a) Silencing of the Sdc 1 expression decreased the number of filopodia/cells, and wild-type-mouse Sdc 1 (wt-mSdc1) could rescue this significant decrease. (b) The silencing of the Sdc 1 expression had no effect on the length of filopodia; however, overexpression of wt-mSdc1 (NS1-wt-mSdc1) as well as the rescue of the Sdc 1 knockdown increased the length of the filopodia. (c, d) Silencing of the Sdc 2 and 4 expression decreased the number of filopodia/cells (c) and the length of the filopodia (d). Expression of wt-mSdc2 or wt-mSdc4, respectively, could rescue these decreases. Overexpression of Sdc 2 (NS2-wt-mSdc2) but not Sdc 4 (NS2-wt-mSdc4) increased the length of the filopodia. Images in Supplemental Figure 1. and comparing NS2 to all other conditions; comparing Sdc 2 KD to Sdc 2 KD rescued with wt-mSdc2; or comparing Sdc 4 KD to Sdc 4 KD rescued with wt-mSdc2. One-way ANOVA with Bonferroni posttest.

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