HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides

Table 3

RT-qPCR analysis of HPV E6/E7 and hTERT mRNA expression in HSIL versus normal clinical specimens.

Cytological diagnosis	HPV E6/E7-positive cases		HPV E6/E7-negative cases
	hTERT- status, N (%)		hTERT- status, N (%)
	Positive	Negative	Positive	Negative
HSIL	80/104 (76.92)	3/104 (2.88)	18/104 (17.31)	3/104 (2.88)
Normal	0/46 (0)	2/46 (4.35)	0/46 (0)	44/46 (95.65)

HPV: human papillomavirus; RT-qPCR: quantitative reverse-transcriptase polymerase chain reaction; HSIL: high-grade squamous intraepithelial lesion.