HPV E6/E7, hTERT, and Ki67 mRNA RT-qPCR Assay for Detecting High-Grade Cervical Lesion with Microscope Slides

Table 4

RT-qPCR analysis of HPV E6/E7 and Ki67 mRNA expression in HSIL versus normal clinical specimens.

Cytological diagnosis	HPV E6/E7-positive cases		HPV E6/E7-negative cases
	Ki67- status, N (%)		Ki67- status, N (%)
	Positive	Negative	Positive	Negative
HSIL	38/104 (36.54)	45/104 (43.27)	11/104 (10.58)	10/104 (9.62)
Normal	0/46 (0)	2/46 (4.35)	1/46 (2.17)	43/46 (93.48)

HPV: human papillomavirus; RT-qPCR: quantitative reverse-transcriptase polymerase chain reaction; HSIL: high-grade squamous intraepithelial lesion.