Research Article
Acylated Ghrelin Renders Chemosensitive Ovarian Cancer Cells Resistant to Cisplatin Chemotherapy via Activation of the PI3K/Akt/mTOR Survival Pathway
Table 1
Antibodies used in the study.
| | Antibody | Cat. No. | MW (kDa) | Manufacturer |
| | GHS-R1a | Sc-374515 | 44, 1 : 1000 | Santa Cruz Biotechnology | | PI3-kinase p85α | sc-1637 | 85 : 1 : 1000 | Santa Cruz Biotechnology | | p-PI3K (Tyr607) | ab182651 | 85 : 1 : 500 | Abcam | | Akt1 | sc-5298 | 62 : 1 : 1000 | Santa Cruz Biotechnology | | p-Akt (Ser473) | 9271 | 60, 1 : 500 | Cell Signaling Technology | | Cleaved caspase-3 | 9661 | 17, 19 : 1 : 250 | Cell Signaling Technology | | BCl-2 | sc-7382 | 26 : 1 : 1000 | Santa Cruz Biotechnology | | Bax | sc-7480 | 23 : 1 : 1000 | Santa Cruz Biotechnology | | NF-κB P65 | sc-8008 | 65, 1 : 500 | Santa Cruz Biotechnology | | p53 | sc-126 | 53 : 1 : 500 | Santa Cruz Biotechnology | | p-p53 (Ser15) | 9248 | 53 : 1 : 250 | Cell Signaling Technology | | mTOR | 2972 | 289 : 1 : 1000 | Cell Signaling Technology | | p-mTOR (Ser2448) | 2971 | 289, 1 : 500 | Cell Signaling Technology | | PUMA | 12450 | 23, 1 : 250 | Cell Signaling Technology | | β-Actin | 4970 | 45, 1 : 1000 | Cell Signaling Technology |
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