Molecular and Cellular Mechanisms Responsible for Beneficial Effects of Mesenchymal Stem Cell-Derived Product “Exo-d-MAPPS” in Attenuation of Chronic Airway Inflammation

Figure 5

Exo-d-MAPPS significantly reduced the total number of inflammatory CD4+ and CD8+ T lymphocytes and increased the presence of immunosuppressive Tregs in inflamed lungs of CS-exposed animals. Representative dot plots showing isotype controls (a). A significantly lower number of CXCR3-expressing and IFN-γ-producing CD4+Th1 cells (b), IL-17-producing CD4+Th17 cells (c), CXCR3-expressing and IFN-γ-producing CD8+CTLs (d), and IL-17- (e) and TNF-α-producing CD8+CTLs (f) were noticed in the lungs of CS-treated mice that received Exo-d-MAPPS, as evidenced by representative dot plots. Exo-d-MAPPS treatment significantly increased the presence of FoxP3-expressing, IL-10-producing CD4+ Tregs in the lungs of CS-exposed animals, as evidenced by representative flow cytometry plots (g). Values are presented as ; mice/group. , , and .