p300-Catalyzed Lysine Crotonylation Promotes the Proliferation, Invasion, and Migration of HeLa Cells via Heterogeneous Nuclear Ribonucleoprotein A1

Figure 2

p300-mediated lysine crotonylation in HeLa cells. (a) p300 expression was measured by western blot in HeLa cells stably expressing nontargeting control (siCon) and sip300. The relative intensities of p300/GAPDH were quantified using ImageJ. . (b) H3 Kcr expression was measured by western blot in HeLa cells stably expressing nontargeting control (siCon) and sip300. The relative intensities of H3 Kcr/H3 were quantified using ImageJ. . (c) Effect of NaCr on H3 Kcr expression was determined by western blot. The relative intensities of H3 Kcr/H3 were quantified using ImageJ. . (d) ACSS2 expression was measured by western blot in HeLa cells stably expressing nontargeting control (siCon) and siACSS2. The relative intensities of ACSS2/GAPDH were quantified using ImageJ. . (e) H3 Kcr expression was determined by western blot in HeLa cell-treated siACSS2 or/and NaCr. The relative intensities of H3 Kcr/H3 were quantified using ImageJ. ; .

(a)

(b)

(c)

(d)

(e)