MicroRNA-342 Promotes the Malignant-Like Phenotype of Endometrial Stromal Cells via Regulation of Annexin A2

Figure 5

miR-342 downregulation inhibits the malignant-like phenotype of endometrial stromal cells by blocking the PI3K/AKT/mTOR signaling pathway. (a, b) The level of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR assessed using Western blot analysis after miR-342 mimic and/or ANXA2 vector. (c, d) The level of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR assessed using Western blot analysis after LY294002 treatment in the presence of miR-342 mimic. (e) Cell proliferation determined using CCK-8 after LY294002 treatment. (f) Cell migration assessed using Transwell migration assay after LY294002 treatment. (g) Cell invasion assessed using Transwell invasion assay after LY294002 treatment. (h) Cell apoptosis determined using flow cytometry after LY294002 treatment. In (a, b), vs. NC mimic + NC vector; ^#vs. miR-342 mimic + NC vector. In (c–h), ^&vs. NC mimic; ^$vs. miR-342 mimic. All data, representative of three independent experiments in triplicate, are shown as . Unpaired -test was adopted to analyze the differences between two experimental groups. Two-way ANOVA, followed by Tukey’s multiple comparison test, was performed to test data among multiple groups. miR: microRNA; ANXA2: Annexin A2; CCK8: cell counting kit-8; NC: negative control; ANOVA: analysis of variance.

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