Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide

<div>Lipopolysaccharide- (LPS-) induced ovarian dysfunction in mice. C57BL/6N female mice (6- to 8-week-old) were intraperitoneally injected with control, low-dose LPS (0.5 mg/kg once daily), high-dose LPS (2.5 mg/kg twice weekly), or cyclophosphamide (CTX; 150 mg/kg once weekly) for 2 weeks. (a) The lengths of estrous cycles were determined by daily observations of vaginal epithelial cell cytology. (b) Hematoxylin and eosin staining was performed to count primordial follicles (black arrow). Scale bar: 100 <i>μ</i>m. (c) Enzyme-linked immunosorbent assay (ELISA) was conducted to measure the levels of serum AMH, E2, and FSH at 2 weeks after treatment. 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Analytical Cellular Pathology

fig1

Figure 1

Figure 1: Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide 