Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide

Figure 3

LPS stimulation induced ovarian fibrosis in mice. (a) Masson staining was performed to detect collagen deposition in mouse ovaries. Representative images are shown. Scale bar: 100 μm. (b) qRT-PCR was performed to determine Col1A1 mRNA expression in the mouse ovaries. (c) Immunohistochemical staining was carried out to detect α-SMA expression in the mouse ovaries. Representative images are shown. Scale bar: 100 μm. (d) Western blotting was performed to determine protein expression of Col1A1 and α-SMA in the mouse ovaries. Data are expressed as the . , , and , . Col1A1: type I procollagen; α-SMA: α-smooth muscle actin.

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