Shikonin Inhibits Non-Small-Cell Lung Cancer H1299 Cell Growth through Survivin Signaling Pathway

Figure 3

Effect of shikonin on cell apoptosis. (a) Shikonin-induced apoptosis in H1299 cells was characterized by nuclear condensation or nuclear fragmentation after Hoechst staining. (b) Flow cytometric analysis of shikonin-induced apoptosis in H1299 cells. The flow cytometry profile presents Annexin V FITC (-axis) and PI staining (-axis). The values represent the percentage of cells in each of the four quadrants (lower left quadrant, viable cells; upper left quadrant, necrotic or dead cells; lower right quadrant, early stage apoptotic cells; and upper right quadrant, late stage apoptotic cells). (c) The mitochondrial membrane potential (Δψm) was assessed using flow cytometry following the treatment of H1299 cells with shikonin (0.78, 1.56, or 3.12 μM) for 48 h.

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