Hsa-miR-105-1 Regulates Cisplatin-Resistance in Ovarian Carcinoma Cells by Targeting ANXA9

Figure 2

ANXA9 was a target gene of Hsa-miRNA-105-1 in OC cells. (a) Schematic diagram showing the complementary sequences in the 3 untranslated region (UTR) of ANXA9 mRNA and seed sequence of Hsa-miRNA-105-1. (b) Luciferase activity (firefly luciferase normalized to Renilla luciferase) in cells transfected with the wild-type or mutant ANXA9 3-UTR reporter construct (, , and paired -test). (c) The levels of ANXA9 mRNA expression in parental and cisplatin-resistant OC cells (, , and paired -test). (d, e) The levels of ANXA9 protein expression in parental and cisplatin-resistant OC cells were detected by western blotting and immunofluorescence. Blue represents the cell nucleus stained with DAPI; green represents ANXA9 positive expression. (f) The effect of ANXA9 expression level on an OC patient’s prognosis as indicated in the Kaplan-Meier Plotter database.

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