Hsa-miR-105-1 Regulates Cisplatin-Resistance in Ovarian Carcinoma Cells by Targeting ANXA9

Figure 4

Downregulation of Hsa-miR-105-1 in OC cells induced resistance to cisplatin. A2780 and TOV-112D cells were transfected with the scramble or Hsa-miR-105-1 inhibitor and then stimulated with cisplatin (1 μg/mL). (a, b) The cell cycle distributions of A2780 and TOV-112D cells as detected by flow cytometry. (c, d) The percentages of apoptotic A2780 and TOV-112D cells were determined by annexin V-FITC/PI staining and flow cytometry. (e) Cell apoptosis was detected by Hoechst staining. (f) The levels of P53, P21, E2F1, Bcl-2, Bax, and caspase-3 protein expression were examined by western blotting. and paired -test.

(a)

(b)

(c)

(d)

(e)

(f)