Ultrasound-Targeted Microbubble Destruction Enhances Inhibitory Effect of Apatinib on Angiogenesis in Triple Negative Breast Carcinoma Xenografts

Figure 6

CD31-PAS double staining of tumor tissues after treatments (×400). (a) Brown granules (CD31-positive staining) were mainly observed in the endothelial cells under a light microscope, and PAS-positive staining was purple-red, showing coarse granular or fine mesh staining in the tumor cells and intercellular space. The IOD of CD31 and PAS was 286.53 and 281.79, respectively. The MV structures composed of endothelial cells with brown cytoplasm could be observed, showing different manifestations of endothelial cell cluster (white arrow) and vascular-like structure (black arrow). The IOD of CD31 and PAS of the MV structures was 183.56 and 5.06, respectively. (b) In a marginal area of the tumor, CD31-positive staining was noted in the endothelial cells, some tumor cells and intercellular space, and PAS-positive staining (purple-red) in the tumor cells and intercellular space showed coarse granular or fine mesh. The IOD of CD31 and PAS was 1601.93 and 1103.91, respectively. A mosaic blood vessel (black arrow) composed of endothelial cells and tumor cells was observed. In the structure, the endothelial cells were weakly positive for CD31, with the IOD of 9.26, and the cell matrix on the luminal surface showed PAS-positive staining, with the IOD of 31.99. White arrow: MV characterized by single endothelial cell.

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