Research Article
Inhibitory Effect of the LY2109761 on the Development of Human Keloid Fibroblasts
Figure 1
LY2109761 inhibits the expression of keloid-derived fibroblast-related factors in vitro. (a) Relative mRNA expression levels of transforming growth factor (TGF)-β1, collagens I and III, fibronectin, and matrix metalloprotease- (MMP-) 1, and MMP-3 as determined by qRT-PCR. (b) Representative western blots showing protein expression of the above gene products. (c) Relative protein expression. (d) ELISA results showing TGF-β1 secretion in response to increasing concentrations of LY2109761. Glyceraldehyde 3-phosphate dehydrogenase (GADPH) was used as the internal control. between LY2109761 groups and DMSO control; between LY2109761 5M and 10 μM treatment groups.
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