TWIST1-EP300 Expedites Gastric Cancer Cell Resistance to Apatinib by Activating the Expression of COL1A2

Figure 1

COL1A2 is highly expressed in GC patients and is associated with poor prognosis and apatinib resistance. (a) Genes with differential expression in the Chen Gastric, Cho Gastric, Cui Gastric, Deng Gastric, and Wang Gastric datasets analyzed by cancer vs. normal analysis in the Oncomine database. (b) Analysis of COL1A2 expression in normal tissues and GC tissues in TCGA-STAD database. (c) Kaplan-Meier analysis of the relationship between COL1A2 and survival in patients with GC in TCGA-STAD database. (d) Analysis of COL1A2 staining intensity in GC tissues versus normal gastric tissues measured using the HPA website. (e) IC50 values of parental and drug-resistant cells examined using the CCK-8 assay. (f) Detection of COL1A2 mRNA expression in parental and drug-resistant cells by RT-qPCR. (g) Detection of COL1A2 protein expression in parental and drug-resistant cells by western blot. Data indicate of three biological replicates. Two-way ANOVA and Tukey’s multiple comparison test; (vs. parental cells).

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