TWIST1-EP300 Expedites Gastric Cancer Cell Resistance to Apatinib by Activating the Expression of COL1A2

Figure 2

TWIST1 is positively correlated with COL1A2 and transcriptionally activates COL1A2. (a) The promoter of COL1A2 was queried in the Ensembl website at Chr7:94394582~94394928. (b) Analysis of the top 30 genes with the highest association with COL1A2 in the Chen Gastric, Cho Gastric, Cui Gastric, Deng Gastric, and Wang Gastric datasets in the Oncomine website. (c) Spearman correlation analysis of TWIST1 with COL1A2 in TCGA-STAD database. (d) The mRNA expression of TWIST1 in parental and drug-resistant cells by RT-qPCR. (e) The protein expression of TWIST1 in parental and drug-resistant cells by western blot. (f, g) The binding of TWIST1 to the COL1A2 promoter in parental cells examined using ChIP-qPCR. (h) Schematic diagram of the pGL3-Luc luciferase reporter vector containing the COL1A2 promoter. (i) The luciferase reporter vector was cotransfected into 293T cells together with different doses of TWIST1 overexpression plasmids to detect changes in luciferase activity in the cells. Data indicate of three biological replicates. Two-way ANOVA and Tukey’s multiple comparison test; (vs. parental cells, IgG, or 0).

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