TWIST1-EP300 Expedites Gastric Cancer Cell Resistance to Apatinib by Activating the Expression of COL1A2

Figure 4

EP300 promotes COL1A2 expression through an H3K27ac-dependent manner. GC cells were treated with HAT inhibitor or DMSO. (a) ChIP-qPCR analysis of the H3K27ac modification level of COL1A2 promoter. (b) The mRNA expression of COL1A2 in parental cells by RT-qPCR. (c) The protein expression of COL1A2 in parental cells by western blot. (d) UCSC browser analysis of the binding of EP300 to the promoter of COL1A2. GC cells were transfected with oe-NC or oe-EP300. (e) ChIP-qPCR analysis of the H3K27ac modification level of COL1A2 promoter. (f) The mRNA expression of COL1A2 in parental cells by RT-qPCR. (g) The protein expression of COL1A2 in parental cells by western blot. (h) Colocalization of TWIST1 with EP300 in AGS and MKN-45 cells by double-labeled immunofluorescence staining. Data indicate of three biological replicates. Two-way ANOVA and Tukey’s multiple comparison test; (vs. DMSO or oe-NC).

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