YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells

Figure 2

YAP expression in HUVECs involved in mediating CM-YAP+ induced tube formation, migration, and proliferation of HUVECs. (a) HUVECs were transfected with YAP CRISPR/Cas9 KO plasmid (YAP KD), YAP expression was detected by Western blot. (b) After starved from FBS for 16 hr, HUVECs, and YAP knock down (KD) HUVECs were treated with different conditioned medium obtained from breast cancer cells for 12 hr. The total segment length and total branching length are quantified with from six independent representative fields , . (c) Migration of HUVECs and YAP knock down HUVECs with different conditioned medium treatment was conducted by Transwell assay. The results are from three independent experiments, . (d) HUVECs and YAP KD HUVECs were treated with different conditioned medium. Cell proliferation was tested by plate cloning formation. The cloning formation rate is quantified with from six independent wells. .

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