Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization

Figure 2

GBC-SD cell-derived exosome-mediated transfer of leptin promotes M2 macrophage polarization. (a) Leptin protein expression in GBC-SD cell derived-exosome was determined by Western blot. (b) The leptin mRNA expression in GBC-SD cell derived-exosome was measured by qRT-PCR. (c) Leptin protein expression in macrophage with PBS or GBC-SD cell-derived exosome-treated was detected by Western blot. The leptin protein expression in macrophage with PBS or GBC-SD cell-derived exosome-treated was quantified. (d) Western blot assay showed leptin protein expression in macrophage treated with exosomes form si-control or si-leptin-transfected GBC-SD cell. The leptin expression in macrophage treated with exosomes form si-control or si-leptin-transfected GBC-SD cell was quantified. (e) qRT-PCR to detect the specific markers for M2-subtype macrophages in macrophage treated with exosomes form si-control or si-leptin-transfected GBC-SD cell. (f) Western blot to detect the specific markers for M2-subtype macrophages in macrophage treated with exosomes form si-control or si-leptin-transfected GBC-SD cell. Data in (a–f) are representative of three independent experiments; the value was determined by Student’s test.

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