Inhibitory Effect of β-Sitosterol on the Ang II-Induced Proliferation of A7r5 Aortic Smooth Muscle Cells

Figure 2

Effects of β-sitosterol on the proliferation, cell cycle progression, and phenotypic switch of Ang II-induced A7r5 cells. (a) Effects of β-sitosterol on the proliferation of Ang II-induced A7r5 cells were detected using the CCK-8 assay. (b) The cell cycle progress of A7r5 cells was tested by flow cytometry. (c) Protein expression of α-SMA, OPN, PCNA, and Cyclin D1 was examined by western blotting. (d) α-SMA, OPN, PCNA and Cyclin D1 protein development gray value analysis histogram. Data indicate the mean ± SD (n = 5, n: number of the sample). Significant differences in different groups compared with the control group (without treatment with Ang II or β-sitosterol) are expressed as , , . Significant differences in different groups compared with the Ang II-alone treatment group are expressed as ^#, ^###, ^###.

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