Nicotine Suppresses Phagocytic Ability of Macrophages by Regulating the miR-296-3p–SIRPα Axis

Figure 2

Nicotine attenuates the phagocytic ability of macrophages by increasing SIRPα expression. (a and b) 48 hours after U937-derived macrophages were transfected with negative control (NC) or si-SIRPα, the levels of SIRPα mRNA and protein were analyzed by qRT-PCR and western blotting, respectively. GAPDH serves as an internal control. (c and d) 24 hours after U937-derived macrophages were transfected with NC or si-SIRPα, nicotine (1 μM) was used to treat the cells for 24 hours. The levels of SIRPα mRNA and protein were analyzed by qRT-PCR and western blotting, respectively. GAPDH serves as an internal control. (e) The phagocytic ability of U937-derived macrophages was analyzed under the same condition. * and **.

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