Hsa_circ_0000190 Promotes NSCLC Cell Resistance to Cisplatin via the Modulation of the miR-1253/IL-6 Axis

Figure 5

IL-6 is a miR-1253 target mRNA. (a) TargetScan identified Il-6 as a candidate miR-1253 target gene. (b) A dual-luciferase reporter assay confirmed the ability of miR-1253 to bind the IL-6 mRNA. (c) IL-6 levels were assessed via qPCR in NSCLC tumor tissues harvested from DDP-resistant (n = 73) and DDP-sensitive (n = 63) patients. (d, e). IL-6 levels were analyzed in NSCLC cell lines, DDP-resistant NSCLC cell lines, and control HBE1 cells via qPCR (d) and ELISA (e). (f, g) Spearman’s correlation coefficients were used to assess relationships between IL-6 expression and the levels of miR-1253 or hsa_circ_0000190. (h, i) IL-6 levels were detected via qPCR and ELISA in NSCLC cells following transfection with si-NC, si-hsa_circ_0000190, si-hsa_circ_0000190 + miR-NC, or si-hsa_circ_0000190 + anti-miR-1253. .

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