Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes
Figure 1
(a) Secondary NOD T cell blasts are resistant to AICD upon tertiary restimulation. NOD or BALB/c Con A blasts were restimulated on anti-CD3-coated plates, washed, and cultured in IL-2-containing medium for 3 days then stimulated again on anti-CD3-coated plates (0.003–3.0 g/mL; 50 l/well). Tritium-labeled thymidine was added for the last 16 hrs of a 72-hour culture. The data are expressed as percent change versus control wells [(mean cpm anti-CD3 wells/mean cpm IgG wells) 100]. The standard deviation of triplicate wells was less than 10%, and the results are representative of four experiments. (b) Culture supernatants were collected at various times (24–96 hrs) following primary stimulation (Con A) of NOD or BALB/c spleen cells or secondary and tertiary stimulations of T cells blasts with plate-bound anti-CD3 (1.0 g/mL). IFN- levels were measured by ELISA, and the results are expressed as ng/mL. The results represent the mean and SD of triplicate wells and are representative of 3 independent experiments. Only -values that are .05 are shown.
