Research Article
Antigen-Experienced CD4lo T Cells Are Linked to Deficient Contraction of the Immune Response in Autoimmune Diabetes
Table 1
The addition of cytokines to the primary stimulation increases the proliferative response in NOD Con A blasts upon secondary challenge with anti-CD3.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Splenocytes were cultured for 72 hrs with 2.0 g/mL of Con A, with or without recombinant mouse IL-2, IL-4, IL-7, or IL-15. Con A blasts were plated in triplicate on IgG-coated (no 20) or anti-CD3-coated plates for 48 hrs, pulsed with H3-thymidine for an additional 18 hrs, harvested, and read in a beta counter. The results are the means, rounded to the nearest 104 CPM. The SD was less than 10%. The asterisk () indicates , when compared to proliferation on IgG coated plate. The stimulation index (SI) was calculated as mean experimental (anti-CD3 plate) CPM/ mean Con A control (IgG plate) CPM. The two asterisks () indicate that when the NOD SI was compared to the SI of NOR blasts treated under the same conditions. Supernatants collected 48 hrs after culture on IgG or anti-CD3-coated plates were tested by ELISA. The results are expressed as mean absorbance (405 nm) of duplicate wells. The absorbance values (1.0–1.54) represent an IFN- concentration range of 1.5–2.5 ng/mL. |