The Effects of Omega-3 Fatty Acids on Matrix Metalloproteinase-9 Production and Cell Migration in Human Immune Cells: Implications for Multiple Sclerosis
Figure 3
Jurkat cell migration is decreased following treatment with DHA or EPA when compared to an untreated control (100% migration). MMP-9 activity from Jurkat cell supernatants is decreased following treatment with DHA or EPA when compared to an untreated control (100% activity). Supernatants were collected from the cultures undergoing migration to determine MMP-9 activity by gelatin substrate zymography. Jurkat cells were incubated for 20 hr in the presence of 10, 30, and 100 μg/mL of DHA or EPA. OA (30 μg/mL) served as an oil control for cell migration. Migration assays were performed three separate times, representative data is displayed. *Significant decrease in migration compared to control (). **Significant decrease in Jurkat cell migration and MMP-9 activity compared to control ().
(a) DHA effect on Jurkat cell migration and MMP-9 Activity
(b) EPA effect on Jurkat cell migration and MMP-9 Activity
