Figure 3: Real-time PCR for EBV genome detection (BamHI-W repeats). (a) Dilution series of DNA (ranging from 0.5 to 105 copies of EBV genome per reaction), extracted from the JY lymphoblastoid cell lines, were analysed as described in Section 2. Real-time PCR resulted in high and constant amplification efficiency for >0.5 copies of EBV genome per reaction. (b) Genomic EBV W repeats were detectable in 6/9 hyperplastic thymuses, 2/5 thymitis, and 4/5 involuted thymuses, but not in two nonpathological control thymuses. Real-time PCR was performed for 50 cycles, and results are expressed as 50-Ct.