213092.fig.004a
(a)
213092.fig.004b
(b)
213092.fig.004c
(c)
213092.fig.004d
(d)
213092.fig.004e
(e)
213092.fig.004f
(f)
213092.fig.004g
(g)
Figure 4: Real-time RT-PCR for the detection of latent EBER1, EBNA1, and LMP1 transcripts. (a, b, c) Analysis of sensitivity of the assay for EBER1 (a), EBNA1 (b), and LMP1 (c) showed that the three EBV latent transcripts could be detected in RNA extracted from a single JY EBV-infected cell. (d, e, f, g) EBER1 (d), EBNA1 (e), and LMP1 (f) were detected in most of the examined MG thymuses but not in normal control thymuses. All MG and control thymuses analysed showed high signals for endogenous control GAPDH amplification (g). Real-time RT-PCR was performed for 50 cycles, and results are expressed as 50-Ct.