Figure 5: Real-time RT-PCR for the detection of lytic BZLF1 transcript. (a) Real-time RT-PCR for BZLF1 could detect the lytic transcript in 7/9 hyperplastic thymuses, 3/5 thymitis, and 5/5 involuted thymuses, but not in normal control thymuses. (b) All MG and control thymuses analysed showed high signals for endogenous control β-actin amplification.