The Caspase Pathway as a Possible Therapeutic Target in Experimental Pemphigus

Figure 3

Mouse skin extracts were analysed using western blot and probed with a monoclonal anticaspase 3 antibody. The first line is a negative control incubated without antibody. The second line corresponds to a skin extract from a mouse injected with normal IgG. Note that a faint band appears at 32 kDa which corresponds to caspase 3 zymogen. The third line corresponds to an animal injected with pemphigus vulgaris IgG and shows a large band of pro-caspase 3 and an additional band at 16 kDa, which corresponds to active caspase 3. The skin extract from the mouse treated with Ac-DEVD-CMK and injected with pemphigus IgG exhibited only the proenzyme.