H11/HspB8 and Its Herpes Simplex Virus Type 2 Homologue ICP10PK Share Functions That Regulate Cell Life/Death Decisions and Human Disease
Figure 4
H11/HspB8 induces TAK1-dependent TNF-α expression. (a) Serial sections from A2058 and A375 xenografts collected at the end of the follow-up period from mice subjected or not to restored H11/HspB8 expression were stained with antibodies to H11/HspB8 (Alexa Fluor 488 conjugated secondary antibody; green) and TNF-α (Alexa Fluor 594 conjugated secondary antibody; red). (b). Serial sections from the A2058 and A375 xenografts as in (a) were stained with Alexa Fluor 488 conjugated NF-κB p65 antibody. Restored H1/HspB8 activates NF-B (intranuclear localization). (c) A375 cells stably transfected with tet-inducible H11/HspB8 were given Dox to restore H11/HspB8 expression alone or with the TAK1 dominant-negative mutant K63W or empty vector. Extracts collected 3 days later were immunoblotted with antibody to pRIP2 (Ser176). They were stripped and reprobed with antibody to actin (loading control). (d) Schematic representation of the pathways involved in H11/HspB8-induced TNF-α induction.
