Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
Figure 1
Recombinant DQ8 and DQ2 proteins with gliadin epitopes stimulate T cell clones. (a) Diagrams of the constructs used to produce recombinant protein for deamidated -gliadin p1E, p9E/DQ8, and -I gliadin/DQ2. The amino acid sequence of the -gliadin peptide in DQ8 is QQYPSGEGSFQPSQENPQ and the -I gliadin peptide (QLQPFPQPELPY) with DQ2. Thrombin, TEV, and PreScission are protease cleavage sites incorporated into the protein constructs. (b) T cell clones restricted to either DQ8 or DQ2 produce IL-2 in response to the deamidated -gliadin/DQ8 or -I gliadin/DQ2 recombinant protein, respectively. (c) The DQ2 and (d) DQ8 T cell responses can be blocked in a dose dependent manner with a monoclonal DQ antibody.
