Differential Immunotoxicity Induced by Two Different Windows of Developmental Trichloroethylene Exposure
Figure 5
TCE altered thymus cellularity in mice exposed to TCE during gestation. Thymocyte suspensions from PND49 mice that had been exposed to TCE during gestation only or during early life only were incubated for 18 hours and then examined by flow cytometry to determine the percentage of single positive, double negative, and double positive cells. In addition, after gating on the double negative cell population, the cells were stained with anti-CD44 and anti-CD25 Abs and labeled as DN1 (CD44+CD25-), DN2 (CD44+CD25+), DN3 (CD44-CD25+), and DN4 (CD44-CD25-). *Significantly different () compared to control values.
