Mucin Reactivity after Acute Exposure to Mercury and Zinc Chloride in Neurocytes of Freshwater Snail Bellamya bengalensis: A Histochemical Study

Table 2

Staining intensity of glycoconjugate in ECM of neuronal cells in control and intoxicated snail B.  bengalensis. AB: alcian blue, PAS: periodic acid, +: moderate reaction, ++: strong reaction, +++: very strong reaction, T: turquoise, and M: magenta.

Heavy metals	Procedure	Staining intensity
		Control	24 hr	48 hr	72 hr	96 hr
HgCl2   induction	AB pH—1.0	+T	++T	++T	+++T	+++T
	AB pH—2.5	+T	++T	+++T	+++T	+++T
	PAS	+++M	++M	+M	+M	+M
	MBB	+++	++	+	+	+
	SBB	+++	++	+	+	+
ZnCl2   induction	AB pH—1.0	+T	+T	++T	++T	+++T
	AB pH—2.5	+T	+T	++T	+++T	+++T
	PAS	+++M	+++M	++M	+M	+M
	MBB	+++	+++	++	++	+
	SBB	+++	+++	++	++	++