In situ localization of EfP-II and EfP-III-1 in the intestine of Eisenia fetida (a) segment 2 as shown in Figure 1. Anti-EfP-II (panel a2) or anti-EfP-III-1 (panel a3) as primary antibodies was added, without adding the primary antibodies as control (panel a1). (b) Segment 5 (see [30]).