Earthworm Protease

Table 4

The Carbohydrate content of the eight isozymes.

Number	Earthworm protease	Carbohydrate content (%)(a)	Relative activity (%)(c)
1	EfP-0-1	6.13	6.2
2	EfP -0-2	4.30	12.8
3	EfP-II-1	6.18	25.8
4	EfP-I-2	1.38	31.6
5	EfP-II-1	1.82	8.8
6	EfP-II-2	7.40	2.1
7	EfP-III-1	6.55	12.5
8	EfP -III-2	4.41	2.3
9	Mixture of isozymes(b)	4.40	100

(a) Mass ratio between the carbohydrate content and the glycoprotein,
(b) Estimated on the fibrin-PAGE by Band-scanned software,
(c)EfPs purified by the SBTI-Sepharose-4B affinity chromatography column. See Wu et al. [26].