Research Article
Comparative Bioremediation of Crude Oil-Amended Tropical Soil Microcosms by Natural Attenuation, Bioaugmentation, or Bioenrichment
Figure 1
Denaturing gradient gel electrophoresis (DGGE) fingerprints of bacterial 16S rRNA gene fragments amplified from Cantagalo (a) and Panelas (b) microcosms submitted to different bioremediation treatments and sampled after 0, 30, 60, 90, and 120 days. Lanes: (1, 6, 11, 16, 21 and 26) 1 Kb ladder, (2) MNA T0, (3) MNAS T0, (4) BE/BA T0, (5) BES/BAS T0, (7) MNA T30, (8) MNAS T30, (9) BE/BA T30, (10) BES/BAS T30, (12) MNA T60, (13) MNAS T60, (14) BE/BA T60, (15) BES/BAS T60, (17) MNA T90, (18) MNAS T90, (19) BE/BA T90, (20) BES/BAS T90, (22) MNA T120, (23) MNAS T120, (24) BE/BA T120, and (25) BES/BAS T120. Strains used for the bacterial consortium: (27) Gordonia alkanivorans DLB 3.22, (28) G. rubriperctinta DTSB 2.5, and (29) Rhodococcus equi DLB 2.6. GelCompar II 4.06 software was used to generate the dendrogram based on the DGGE clustering profiles of the soils from Cantagalo (c) and Panelas (d) using Pearson’s coefficient and the clustering method of UPGMA.
