Capacity of Histoplasma capsulatum to Survive the Composting Process
Table 1
Trials of the composting reactors system leading to establish survival of H. capsulatum at the composting process after 45 days.
Contaminated reactor
Control reactor
Observations
Assay no.
Inoculum (CFU/ml)†
Depth
Culture on mycosel
Hc100 nested PCR
NTC 5167
1
4200
Not done
Negative
Positive
Approved
Flies grew inside culture media; this situation lead to an excessive grow of bacteria in culture, which did not allow to visualize the H. capsulatum growth.
2
3800
Not done
Negative
Positive
Approved
Bacteria and other fungi had an abundant growth, which did not allow to visualize the H. capsulatum growth.
3
5800
Surface
Negative
Positive
Approved
5 cm
Negative
Negative
10 cm
Negative
Positive
15 cm
Negative
Positive
4
4050
Surface
Negative
Positive
Approved
Microbiological study was carried out to the satisfaction, although the growth of H. capsulatum was not obtained.
5 cm
Negative
Positive
10 cm
Negative
Positive
15 cm
Negative
Negative
5
3200
Surface
Negative
Positive
Approved
5 cm
Negative
Negative
10 cm
Negative
Positive
15 cm
Negative
Positive
†Quantification of H. capsulatum inoculum solution which was used to contaminate each reactor.