| Stage | Primary variables | Secondary variables | Measurement date |
| Characterization | Total content (HMtot) | Four physicochemical variables (pH, EC, TOC, and TN) | February 13, 2014 (T111/T240) |
| Evaluation | Total content (HMtot), Bioavailability (HMDTPA) and solubility (HMH2O) | Eleven physicochemical variables (T°, H%, pH, EC, TOC, TN, NH4+-N, HA, FA, OM, and Da) | February 2, 2016 (T0); | Twelve microbiological variables (mesophilic aerobes, thermophilic aerobes, Lactobacillus spp., molds and yeasts, total coliforms, fecal coliforms, Escherichia coli, Salmonella spp., Pseudomonas spp., Bacillus spp., actinomycetes, and helminths) | March 3, 2016 (T30); April 2, 2016 (T60); May 2, 2016 (T90); June 1, 2016 (T120) |
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