Advances in Hematology / 2009 / Article / Fig 1

Research Article

Functional Characterization of the Canine Heme-Regulated eIF2 Kinase: Regulation of Protein Synthesis

Figure 1

(a) Expression pattern of HRI in human tissues. RT-PCR products from human tissue using HRI specific primers were separated on a 0.8% agarose gel and visualized by UV. Lanes: 1, liver; 2, kidney; 3, lymph node; 4, spleen. (b) RT-PCR products of canine HRI cDNA from spleen. 50 ng of the resulting cDNA PCR products were separated on an agarose gel (0.8%), stained with ethidium bromide and visualized by UV. (c) Nucleotide sequence comparison of the canine HRI with human, rat and mouse HRI. Conserved nucleotides between all four species are denoted with a star. (d) Amino acid sequence alignment of the canine HRI with the mouse, rat, and human HRI. Semi-conserved residues are designated with a period underneath, conserved residues are shown by semicolon underneath, and the identical residues have stars underneath them. The residues important for ATP binding are highlighted in gray. (e) Alignment of the residues involved in ATP binding. Residues important for ATP binding for the human, mouse, rat, and canine HRI are shown. Divergences between the species are highlighted.
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