Functional Characterization of the Canine Heme-Regulated eIF2 Kinase: Regulation of Protein Synthesis
Figure 3
(a) Restriction digestion analysis of the canine HRI-bacmid DNA verifies the insertion of canine HRI cDNA into the bacmid DNA with correct orientation. Lane 1, Molecular Weight markers; Lane 2, pFastBac-canine HRI cDNA. (b) Expression of mammalian HRIs. HRI, expressed in insect cells, was detected by Western blot analysis using an affinity purified mouse monoclonal antibody against histidine and visualized by enhanced chemiluminescence. Lane 1, canine HRI; Lane 2, mouse HRI; Lane 3, rat HRI; Lane 4, human HRI.
