Screening for Residual Disease in Pediatric Burkitt Lymphoma Using Consensus Primer Pools

Figure 1

Examples of melt curve analyses with corresponding ethidium-stained amplified products. Melt curves displayed as −dF/dT plots: (a) defined peak with minimal to no shoulder indicates a positive MRD result; (b) a broad peak indicates a negative result; (c) a defined peak with significant shoulders indicates equivocal results. Representative 2.0% agarose gels are at the left of each of graph. Melt curves of negative controls: (d) PBMC from normal healthy individual shows a broad undefined peak; (e) water (no template) shows no peak; (f) melt curve assessment of PCR products: melt curve peak shift disparities are noted between diagnostic and followup specimens, consistent with possible different clonal differentiation over time.