and : Critical Residues for In Vitro Biological Activity of Reteplase
Figure 6
(a) Protease activity assay for rPA and its mutants. Sample 1 is WT (wild type rPA gene); sample 2: N12P, N278S double mutant; sample 3: N12P mutant; sample 4: N278S mutant; sample 5: commercial retavase preparation. (b) Clot lysis activity by turbidimetric fibrinolytic assay. axis shows incubation time in minutes for clot lysis while axis shows % clot lysis. The horizontal line indicates 50% clot lysis while the vertical dotted lines indicate the t 1/2 values of different rPA samples. The course of clot lysis is demonstrated by connecting data points of different time intervals. (c) Plasminogen activation assay. axis shows sample types while axis shows absorbance at 405 nm. Sample 1: WT rPA; sample 2: N12P, N278S double mutant; sample 3: N12P mutant; sample 4: N278S mutant; sample 5: commercial retavase preparation.
