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Advances in Hematology
Volume 2010, Article ID 896161, 8 pages
http://dx.doi.org/10.1155/2010/896161
Research Article

Photodynamic Therapy with the Silicon Phthalocyanine Pc 4 Induces Apoptosis in Mycosis Fungoides and Sezary Syndrome

1Department of Dermatology, University Hospitals of Cleveland, Case Western Reserve University, 11100 Euclid Avenue, Lakeside 3500, Cleveland, OH 44106-5028, USA
2Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China
3Department of Radiation Oncology, Case Western Reserve University, 10900 Euclid Ave., Cleveland, OH 44106-4942, USA
4Dermatology Department, Cleveland Veterans Affairs Medical Center, Cleveland, OH 44106, USA

Received 6 July 2010; Revised 2 September 2010; Accepted 14 October 2010

Academic Editor: Christian Peschel

Copyright © 2010 Minh Lam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Our current focus on the effects of Photodynamic Therapy (PDT) using silicon phthalocyanine Pc 4 photosensitizer on malignant T lymphocytes arose due to preclinical observations that Jurkat cells, common surrogate for human T cell lymphoma, were more sensitive to Pc 4-PDT-induced killing than epidermoid carcinoma A431 cells. Mycosis fungoides (MF) as well as Sezary syndrome (SS) are variants of cutaneous T-cell lymphoma (CTCL) in which malignant T-cells invade the epidermis. In this study, we investigated the cytotoxicity of Pc 4-PDT in peripheral blood cells obtained from patients with SS and in skin biopsies of patients with MF. Our data suggest that Pc 4-PDT preferentially induces apoptosis of CD4+CD7 malignant T-lymphocytes in the blood relative to CD11b+ monocytes and nonmalignant T-cells. In vivo Pc 4-PDT of MF skin also photodamages the antiapoptotic protein Bcl-2.