Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion
Figure 2
Tissue distribution of MSCs in vivo after injection. A. Bioluminescent imaging (BLI) of MSCs at 4, 7, and 24 hours after injection. MSC cells stably expressing ffluc were infused into recipient mice by tail vein injection followed by Bioluminescent signals were restricted to the thoracic area. Signals decreased rapidly and were undetectable 72 hours after infusion. B. Quantitative RT-PCR for ffluc in the above tissues harvested at 1, 4, and 7 days after infusion ( each). Whole RNA was prepared from tissues and analyzed for presence of ffluc mRNA by q RT-PCR. Since ffluc expression cannot be normalized to any endogenous control, equal quantities of starting material (2 μg of whole RNA) were used for cDNA synthesis. Those samples with mean Ct < 35 cycles were deemed as positive for ffluc expression. All animals had detectable levels of ffluc mRNA in their lungs on day 1, while most other tissues had no detectable transcript on day 1. On days 4 and 7, there were detectable levels of ffluc transcripts detectable in a variable proportion of all tissues from some animals.
