Engineered T Cells for the Adoptive Therapy of B-Cell Chronic Lymphocytic Leukaemia

Figure 3

ZAP70-positive B-CLL cells are more efficiently eliminated by CAR-redirected T cells in vitro. The efficacy in specific cytolysis by CAR-redirected T cells (data from Figure 1(d)) was plotted against (a) serum thymidine kinase-1 levels (<10 U/L versus >10 U/L), (b) mutated versus unmutated status of the immunoglobulin heavy chain variable region of B-CLL cells, and (c) patient’s age (<70 yrs versus >70 yrs). Closed circles represent CD3ζ CAR, open circles CD28-CD3ζ CAR-mediated B-CLL killing. Depletion from CD25^high Treg cells improves redirected cytolysis of B-CLL cells as exemplarily shown for two patients. Statistic calculations were performed using Student’s 𝑡-test, *𝑃<0.05; **𝑃<0.001.