Genomic Amplification of an Endogenous Retrovirus in Zebrafish T-Cell Malignancies
ZFERV amplifications in hlk zebrafish. DNA from 6 hlk fish with normal phenotype and 3 with GFP+ cancers was tested by qPCR of pol (a) and env (b). White bars show tail DNA of individual normal (lanes 3–8) or T-ALL+ (lanes 13–15) fish. All were statistically similar to each other and to WT Tails 7–9 (lane 1). Pooled T cells from hlk fish without T-ALL (gray bars) had ZFERV gains comparable to normal WIK T cells (lane 2). Mean copy number was higher in hlk T cells than tails (lane 9 versus 12) in the same animals. Diagonally striped bars show amplifications in neoplastic T cells of 3 hlk fish (lanes 16–18). Mean copy gains were similar in non-malignant and malignant hlk T cells (lane 12 versus 19). Other details are as described in the legend to Figure 3.
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