Genomic Amplification of an Endogenous Retrovirus in Zebrafish T-Cell Malignancies
ZFERV amplifications in two other D. rerio T-cell cancers. One WT WIK fish spontaneously developed a GFP+ thymic tumor and was tested by qPCR of pol (a) and env (b). Average copy numbers of other samples tested previously are shown as black bars. Germline ZFERV copy number in this fish (lane 9) was similar to the 15 WIK fish already examined (lane 1). This tumor showed 5.5-fold amplification (lane 10, diagonal bar), similar to non-malignant T cells and cancers from WT, hlk, and MYC-ER fish (lanes 4–8). One other hlk T-ALL exhibited high-level, 25- to 30-fold gains (lane 12), although its germline copy number (lane 11) was comparable to other fish (lanes 1–3).
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