Advances in Hematology / 2012 / Article / Fig 2

Research Article

In Vitro and In Vivo Antitumor Effect of Anti-CD33 Chimeric Receptor-Expressing EBV-CTL against Acute Myeloid Leukemia

Figure 2

Anti-CD33.CAR-transduced EBV-CTLs efficiently killed autologous LCLs and CD33+ KG-1 cells. Cytotoxicity of unmanipulated EBV-CTLs and anti-CD33.CAR-transduced EBV-CTLs was evaluated by a standard 4-hour 51Chromium-release assay after 7 days of culture at effector:target (E : T) ratios of 50 : 1, 25 : 1, 10 : 1 and 5 : 1 against autologous LCLs (a) and CD33+ KG-1 cell line (b). To assess the specificity of either HLA-I-mediated and CAR-induced killing, target cells were preincubated with anti-human HLA-I (a) antibodies or an anti-human CD33 monoclonal antibody (b) before the addition of the effector cells. Data shown are mean ± SD of 6 separate experiments; .

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