Advances in Hematology / 2012 / Article / Fig 3

Research Article

In Vitro and In Vivo Antitumor Effect of Anti-CD33 Chimeric Receptor-Expressing EBV-CTL against Acute Myeloid Leukemia

Figure 3

Anti-CD33.CAR-transduced EBV-CTLs released substantial levels of IFN-γ and Granzyme B when stimulated with either autologous LCLs and CD33+ KG-1 cells. Release of IFN-γ (a) and Granzyme B (b) from unmanipulated or anti-CD33.CAR-transduced EBV-CTLs was evaluated by ELISpot assays after stimulation with either irradiated autologous LCLs or irradiated KG-1 cells at 1 : 2 ratio. To assess the specificity of either HLA-I-mediated and CAR-induced cytokine release target cells were preincubated with anti-human HLA-I antibodies or an anti-human CD33 monoclonal antibody before the addition of the effector cells. Data shown are mean ± SD of 6 separate experiments; ; .

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